The Aquilos 2 is a dual beam FIB-SEM platform specifically designed for sectioning of cryo-fixed samples, producing thin lamellae suitable for cryo-electron tomography at an TEM in an automated batch method. Updated with the integrated fluorescence microscopy module – METEOR system and CERES ice shield (Delmic), our Aquilos 2 allows for performing in-situ fluorescence microscopy directly on the sample inside the system and reduces transfer steps and associated ice contamination, simultaneously increasing the efficiency of the cryo- correlative light and electron microscopy (CLEM) pipeline. The Aquilos 2 can be used to cut “windows” in (biological specimen) so cellular processes can be subsequently visualized and later resolved three-dimensionally in their native environment.